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目錄:北京索萊寶科技有限公司>>抗體>>內(nèi)參抗體>> M1001060anti-Flag Tag Mouse Monoclonal antibody(鼠單克隆抗體)

anti-Flag Tag Mouse Monoclonal antibody(鼠單克隆抗體)
  • anti-Flag Tag Mouse Monoclonal antibody(鼠單克隆抗體)
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  • 品牌 SOLARBIO/索萊寶
  • 型號(hào) M1001060
  • 廠商性質(zhì) 生產(chǎn)商
  • 產(chǎn)品資料 查看pdf文檔
  • 所在地 北京市
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更新時(shí)間:2024-08-25 15:49:06瀏覽次數(shù):2070評(píng)價(jià)

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anti-Flag Tag Mouse Monoclonal antibody(鼠單克隆抗體)在Western Blotting中使用內(nèi)參其實(shí)就是在WB過(guò)程中的另外用內(nèi)參對(duì)應(yīng)的抗體檢測(cè)內(nèi)參,這樣在檢測(cè)目的產(chǎn)物的同時(shí)可以檢測(cè)內(nèi)參的表達(dá),由于內(nèi)參在各組織和細(xì)胞中的表達(dá)相對(duì)恒定,借助檢測(cè)每個(gè)樣品內(nèi)參的量就可以用于校正上樣誤差,這樣半定量的結(jié)果才更為可信。

英文名稱      anti-Flag Tag Mouse Monoclonal antibody

應(yīng)用             WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.

規(guī)  格           100ul

濃  度           1mg/ml

保  存           Store at -20°C. Do not aliquot the antibody.

儲(chǔ)存液        PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

純化方法     affinity purified by Protein A

亞  型           IgG

性  狀           Liquid

克隆類型      monoclonal

宿  主           Mouse

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

說(shuō)明書(shū)

Anti- Flag Tag Mouse Monoclonal Antibody (1B3)

Catalog No. M1001060

Size100μl

Storage: Store at -20°C. Do not aliquot the antibody.

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to

interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively

as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to

separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can

also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many

different assays that require recognition by an antibody. If there is no antibody against the studied protein,

adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag

sequence.

Molecular Weight: N/A

Application: WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.

Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag

fusion proteins.

Form: Liquid ,1mg/ml

Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

Relative Products

M1001010 Anti- HA Tag Mouse Monoclonal Antibody(2C7)

M1001020 Anti-His Tag Mouse Monoclonal Antibody(3C5)

M1001030 Anti-GFP Tag Mouse Monoclonal Antibody(2H7)

M1001040 Anti-GST Tag Mouse Monoclonal Antibody(2C7)

M1001050 Anti- cMyc Tag Mouse Monoclonal Antibody(2C5)

M1001060 Anti- Flag Tag Mouse Monoclonal Antibody(1B3)

RG001010 Anti- HA Tag Rabbit Polyclonal Antibody

RG001020 Anti- His Tag Rabbit Polyclonal Antibody

RG001030 Anti- GFP Tag Rabbit Polyclonal Antibody

RG001040 Anti- GST Tag Rabbit Polyclonal Antibody

RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody

RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody

Solarbio

Beijing Solarbio Science & Technology Co., Ltd

/82

Http://www.solarbio。。com

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