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After the primary antibody binds to the target protein, it forms a complex with the HRP-conjugated secondary antibody. Add LumiGLO*, which emits light during enzyme-catalyzed breakdown.

Application Keywords:
WB - Western blot IP -immunoprecipitation IHC - Immunohistochemistry ChIP -chromatin immunoprecipitation IF - Immunofluorescence F - Flow Cytometry EP -ELISA Peptides

Species cross-reactivity keywords:
H - person M -mouse R - rat Hm - Hamster Mk - Monkey Vir - Virus Mi - Mink C - Chicken Dm - Drosophila melanogaster X - Xenopus Z -zebrafish B - cattle DG - Canine PG - Pig Sc - Saccharomyces cerevisiae Ce - Caenorhabditis elegans Hr - Horse All - All species are expected

background

Chemiluminescence systems have emerged as one of the most comprehensive Western blot detection methods. They avoid the dangers associated with radioactive substances and toxic chromogenic substrates. The detection speed and sensitivity of these methods are unmatched by traditional methods, and the results are produced on film, so the data can be permanently recorded and stored. Blots detected with chemiluminescence can be eluted so that they can be subsequently re-detected with other antibodies. HRP-conjugated secondary antibodies are combined with specific chemiluminescent substrates to generate a light signal. The HRP conjugate has a high turnover rate, resulting in good sensitivity in a short reaction time.