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CYT-421

重組人干細胞因子（sf-9來源）（rHuSCF-Sf9）

2μg/10μg/1mg

Prospec

Description :
	Recombinant Human SCF produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. SCF is fused to a C-terminal His-tag （6x His） and purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	Lyophilized from a concentrated （1mg/ml） solution in water containing no additives.
	Solubility:
	It is recommended to reconstitute the lyophilized Recombinant Human SCF in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Recombinant Human SCF should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
	Purity:
	Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec’s Recombinant SCF-Sf9 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 5x10⁵ IU/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of SCF-Sf9.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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