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CYT-261

重組人骨形態(tài)發(fā)生蛋白2（rHuBMP-2）

2μg/10μg/1mg

Prospec

Background :
	Belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta （TGF-beta） superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
	Description :
	Recombinant Human Bone Morphogenetic protein-2 produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton. BMP-2 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	BMP-2 was lyophilized from a concentrated （1mg/ml） sterile solution containing 10mM sodium citrate pH=3.5.
	Solubility:
	It is recommended to reconstitute the lyophilized rhBMP-2 in sterile 20mM AcOH （acetic Acid） not less than 100µg/ml, which can then be further diluted to other aqueous solutions
	Stability:
	Lyophilized BMP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec's BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less then 50 ng/ml.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of Bone Morphogenetic protein-2 .
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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