目錄:愛必信（上海）生物科技有限公司>>細(xì)胞生物學(xué)>>重組蛋白>> abs00996Recombinant Mouse M-CSF（2ug）
| 供貨周期 | 一周 | 規(guī)格 | 2ug |
|---|---|---|---|
| 貨號(hào) | abs00996 |
| 概述 | |
|---|---|
| 種屬 | Murine |
| 形態(tài) | Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4. |
| 來源 | Escherichia coli. |
| 氨基酸序列 | KEVSEHCSHM IGNGHLKVLLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE |
| 內(nèi)毒素水平 | Less than 1 EU/μg of rMuM-CSF as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| Accession # | Q3U4F9 |
| 預(yù)期分子量 | Approximay 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 95 % by SDS-PAGE and HPLC analyses. |
| 參考文獻(xiàn) | |
|---|---|
| 1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.<br/>2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52.<br/>3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35.<br/>4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102. | |
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