目錄:愛必信（上海）生物科技有限公司>>細(xì)胞生物學(xué)>>重組蛋白>> abs00877Recombinant Human bFGF
| 供貨周期 | 一周 | 規(guī)格 | 10ug |
|---|---|---|---|
| 貨號 | abs00877 |
| 概述 | |
|---|---|
| 種屬 | Human |
| 形態(tài) | Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl. |
| 來源 | Escherichia coli. |
| 氨基酸序列 | MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS |
| 內(nèi)毒素水平 | Less than 1 EU/µg of Recombinant HumanbFGF as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
| Accession # | P09038 |
| 預(yù)期分子量 | Approximay 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 96 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 2247 |
| 參考文獻(xiàn) | |
|---|---|
| 1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.<br/>2. Gospodarowicz D. 1974. Nature. 249:123-7.<br/>3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.<br/>4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.<br/>6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.<br/>7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9. | |
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