Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) 

谷氨酸半胱氨酸連接酶催化亞基(GCLC)重組蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Met429~Asn637

Tags: N-terminal His Tag

Subcellular Location: Secreted

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation:PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 400µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:4.9

Predicted Molecular Mass:25.1kDa

Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

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