欧美……一区二区三区,欧美日韩亚洲另类视频,亚洲国产欧美日韩中字,日本一区二区三区dvd视频在线

您好, 歡迎來(lái)到化工儀器網(wǎng)

| 注冊(cè)| 產(chǎn)品展廳| 收藏該商鋪

17801761073

products

目錄:北京索萊寶科技有限公司>>ELISA試劑盒>>人 Human ELISA>> 1600/48T; 2600/9Human IL-4 人白介4

Human IL-4 人白介4
  • Human IL-4 人白介4
  • Human IL-4 人白介4
參考價(jià) 面議
具體成交價(jià)以合同協(xié)議為準(zhǔn)
參考價(jià) 面議
具體成交價(jià)以合同協(xié)議為準(zhǔn)
  • 品牌 SOLARBIO/索萊寶
  • 型號(hào) 1600/48T; 2600/9
  • 廠商性質(zhì) 生產(chǎn)商
  • 所在地 北京市
屬性

$NV_PropertyInfoName.SubString(0,25)

>

更新時(shí)間:2024-08-26 11:24:56瀏覽次數(shù):2981評(píng)價(jià)

聯(lián)系我們時(shí)請(qǐng)說(shuō)明是化工儀器網(wǎng)上看到的信息,謝謝!

同類優(yōu)質(zhì)產(chǎn)品

更多產(chǎn)品
供貨周期 現(xiàn)貨 規(guī)格 1600/48T; 2600/96T
貨號(hào) SEKH-0011 應(yīng)用領(lǐng)域 生物產(chǎn)業(yè)
主要用途 檢測(cè)人血清/血漿/細(xì)胞培養(yǎng)上清中的白介素4的含量,定量分析
Human IL-4 人白介4定量檢測(cè)
索萊寶ELISA試劑盒的優(yōu)勢(shì):
1,包被的酶標(biāo)板單板可拆(能拆分成12個(gè)8孔的酶標(biāo)條)
2,提供免費(fèi)代測(cè)代檢服務(wù),代出實(shí)驗(yàn)數(shù)據(jù)
3,發(fā)文章高獎(jiǎng)勵(lì)
4,磁鐵可吸附式包裝盒,包裝精美耐用

 For research use only. Not for use in diagnostic procedures.

Human IL-4 人白介4?

MANUFACTURED AND DISTRIBUTED BY:

CountryCompany: ChinaBeijing Solarbio Science & Technology Co., Ltd

Address: NO.8, Liandong U Valley, Tongzhou District, Beijing, P.R.China.

     

 

TABLE OF CONTENTS

 

SECTION                                          PAGE                                                                    

BACKGROUND...........................................................................................3

PRINCIPLE OF THE ASSAY......................................................................3

TECHNICAL HINTS AND LIMITATIONS...............................................4

PRECAUTIONS............................................................................................4

KIT COMPONENTS& STORAGE CONDITIONS.....................................5

OTHER SUPPLIES REQUIRED BUT NOT SUPPLIED............................6

SPECIMEN COLLECTION & STORAGE..................................................6

REAGENTS PREPARATION......................................................................6

ASSAY PROCEDURE .................................................................................8

CALCULATION OF RESULTS...................................................................8

PERFORMANCE CHARACTERISTICS....................................................10

REFERENCES..............................................................................................11

Human IL-4 人白介4?

BACKGROUND

The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell.It is closely related and has functions similar to Interleukin 13.IL-4 has been shown to drive mitogenesis, dedifferentiation, and metastasis in rhabdomyosarcoma.IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma.

 

PRINCIPLE OF THE ASSAY

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IL-4 is added to detect the captured IL-4 protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

 

TECHNICAL HINTS AND LIMITATIONS

  • This Solarbio ELISA should not be used beyond the expiration data on the kit label.
  • To avoid cross-contamination, use a fresh reagent reservoir and pipette tips for each step.
  • To ensure accurate results, some details, such as technique, plasticware and water sources should be emphasized.
  • A thorough and consistent wash technique is essential for proper assay performance.
  • A standard curve should be generated for each set of samples assayed. 
  • It is recommended that all standards and samples be assayed in duplicate.
  • Avoid microbial contamination of reagents and buffers. Buffers containing protein should be made under aseptic conditions and be prepared fresh daily.
  • In order to ensure the accuracy of the results, the standard curve should be made every time.

PRECAUTIONS

The Stop Solution suggested for use with this kit is an acid solution. Wear protective gloves, clothing, eye, and face protection. Wash hands thoroughly after handling.

KIT COMPONENTS& STORAGE CONDITIONS

PART

SIZE

STORAGE OF OPENED/ RECONSTITUTED MATERIAL

Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips)

1 plate

Return unused wells to the foil pouch containing the desiccant pack. Reseal along entire edge of the zip-seal. May be stored for up to 1 month at 2 – 8℃**

Standard - lyophilized,1000 pg/ml upon reconstitution

2 vials

Aliquot and Store at -20°C** for six months

Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial

1 vial

Store at 2-8°C **for six months

Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial

1 vial

Store at 2-8°C** for six months

Standard /sample Diluent - 16 ml/vial

1 bottle

Store at 2-8°C** for six months

Biotin-Conjugate antibody Diluent - 16 ml/vial

1 bottle

Store at 2-8°C** for six months

Streptavidin-HRP Diluent - 16 ml/vial

1 bottle

Store at 2-8°C** for six months

Wash Buffer Concentrate (20x) - 30 ml/vial

1 bottle

Store at 2-8°C** for six months

Substrate Solution - 12 ml/vial

1 bottle

Store at 2-8°C** for six months

Stop Solution - 12 ml/vial

1 bottle

Store at 2-8°C** for six months

Plate Cover Seals

 4 pieces

 

 

**Provided this is within the expiration date of the kit.

OTHER SUPPLIES REQUIRED BUT NOT SUPPLIED

  • Microplate reader capable of measuring absorbance at 450 nm.
  • Pipettes and pipette tips.
  • Deionized or distilled water.
  • Squirt bottle, manifold dispenser, or automated microplate washer.
  • 500 mL graduated cylinder.
  • Human IL-4 controls (optional; available from Solarbio).

 

SPECIMEN COLLECTION & STORAGE

Cell Culture Supernates - Centrifuge cell culture media at 1000×g to remove debris. Assay immediay or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles.

Serum - Use a serum separator tube (SST) and allow samples to clot for 2 hours at room temperature or overnight at 2-8℃. Centrifuge at approximay for 15 minutes at 1000×g. Assay immediay or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles.

Plasma - Collect plasma using EDTA, heparin, or citrate as an anticoagulant. Centrifuge for 15 minutes at 1000×g within 30 minutes of collection. Assay immediay or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles.

Note: The normal human serum or plasma samples are suggested to make a 1:2 dilution.

 

REAGENTS PREPARATION

  • Temperature returning - Bring all kit components and specimen to room temperature (20-25) before use.
  • Wash Buffer - Dilute 30mL of Wash Buffer Concentrate with 570mL of deionized or distilled water to prepare 600mL of Wash Buffer. If crystals have formed in the concentrate Wash Buffer, warm to room temperature and mix gently until the crystals have compley dissolved.
  • StandardSpecimen - Reconstitute the Standard with 1.0mL of deionized or distilled water. This reconstitution produces a stock solution of 1000pg/mL. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 800mL of Standard/Specimen Diluent into the 200 pg/mL tube, and add 200mL stock solution of 1000 pg/mL into it to get the high standard of 200 pg/mL. Pipette 500mL of Standard/Specimen Diluent into the remaining tubes. Use the high standard to produce a 2-fold dilution series (below).. Mix each tube thoroughly and change pipette tips between each transfer. The 200pg/mL standard serves as the high standard. The Standard/specimen Diluent serves as the zero standard (0pg/mL).

*If you do not run out of re-melting standard, store it at -20. Diluted standard shall not be reused.

  • Working solution of Biotin-Conjugate anti-human IL-4 antibody: Make a 1:100 dilution of the concentrated Biotin-Conjugate solution with the Biotin-Conjugate antibody Diluent in a clean plastic tube.

*The working solution should be used within one day after dilution.

  • Working solution of Streptavidin-HRP: Make a 1:100 dilution of the concentrated Streptavidin-HRP solution with the Streptavidin-HRP Diluent in a clean plastic tube. 

*The working solution should be used within one day after dilution.

 

Preparation of IL-4 standard dilutions

 

ASSAY PROCEDURE

 CALCULATION OF RESULTS

  • The standard curve is used to determine the amount of specimens.
  • First, average the duplicate readings for each standard, control, and sample. All O.D. values are subtracted by the mean value of blank control before result interpretation.
  • Construct a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph.
  • The data may be linearized by plotting the log of the IL-4 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
  • This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Typical data using the IL-4 ELISA

Standard(pg/ml)

OD.

OD.

Average

Corrected

0

0.044

0.04

0.042

    ------

3.12

0.097

0.092

0.0945

0.0525

6.25

0.13

0.106

0.118

0.076

12.5

0.229

0.233

0.231

0.189

25

0.42

0.412

0.416

0.374

50

0.741

0.734

0.7375

0.6955

100

1.315

1.306

1.3105

1.2685

200

2.205

2.212

2.2085

2.1665

 

Representative standard curve for IL-4 ELISA.

Performance Characteristics

SENSITIVITY: The minimum detectable dose was 1.5pg/mL.

SPECIFICITY: This assay recognizes both natural and recombinant human IL-4. The factors listed below were prepared at 100ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.

 Factors assayed for cross-reactivity

Recombinant human

Recombinant mouse

Recombinant porcine

 G-CSF

IL-1

bovine FGF acidic

  GM-CSF

IL-4

bovine FGF basic

  IL-1α_

IL-3

human PDGF

  IL-1β_

IL-4

porcine PDGF

   IL-2 sRα_

IL-5

 

IL-3

 

 

IL-4

 

 

IL-6

 

 

IL-7

 

 

IL-8

 

 

LIF

 

 

TGF-β1

 

 

TGF-β2

 

 

REPEATABILITY: The coefficient of variation of both intra-assay and inter-assay were less than 10%.

RECOVERY:The recovery of IL-4 spiked to three different levels in four samples throughout the range of the assay in various matrices was evaluated.

 Recovery of IL-4 in two matrices  

Sample Type

Average % of Expected Range (%)

Range (%)

Citrate plasma

88

82-94

Cell culture supernatants

97

92-102

LINEARITY:To assess the linearity of the assay, three samples were spiked with high concentrations of IL-4 in various matrices and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

 The linearity of the assay

Dilution ratio

Recovery (%)

Citrate plasma

Cell culture supernatants

1:2

Average% of Expected

85

96

Range (%)

81-89

92-100

1:4

Average% of Expected

92

102

Range (%)

87-97

96-108

REFERENCES

  • Sokol, C.L., Barton, G.M., Farr, A.G. & Medzhitov, R. (2008). "A mechanism for the initiation of allergen-induced T helper type 2 responses". Nat Immunol 9 (3): 310–318.
  • Liang, H-E, et al. (2012) Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nature Immunology, 13: 58–66.
  • Howard M, Paul WE (1982). "Interleukins for B lymphocytes". Lymphokine Res. 1 (1): 1–4.
  • Yokota T et al. (1986). "Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that express

 

 

 

 

 

會(huì)員登錄

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
在線留言

會(huì)員登錄

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:
熱線電話 在線詢價(jià)
欧洲精品一区二区三区中文字幕-91久久国产综合久久蜜月精品| 国模自慰一区二区三区-日韩一级黄色片天天看| hd在线观看一区二区-免费一区二区三区毛片在线| 性色国产成人久久久精品二区三区-偷窥中国美女洗澡视频| 久久精品亚洲国产av久-国产精品视频一区二区免费| 午夜福利卫生纸福利院-一区二区三区久久亚洲| 俄罗斯胖老太太黄色特级片-国产精品黑丝美腿美臀| 国产黄片在现免费观看-色老板最新在线播放一区二区三区| 婷婷综合在线视频观看-亚洲一区二区三区香蕉| 人妻丝袜中文字幕在线视频-亚洲成av人片一区二区三区| 国产福利视频一区二区三区-日韩人妻中文视频精品| 人妻日韩精品中文字幕图片-麻豆极度性感诱人在线露脸| 男人的天堂久久精品激情-最新亚洲精品a国产播放| 丝袜美腿人妻连续中出-在线观看日韩三级视频| 韩漫一区二区在线观看-精品国产免费未成女一区二区三区| 午夜福利院免费在线观看-久久精品日产第一区二区三区画质| 国产在线不卡高清一区-日本一区二区三区四区无卡| 国内精产熟女自线一二三区-六月丁香婷婷在线观看| 国产欧美一区二区三区嗯嗯-欧美一区二区日本国产激情| 亚洲欧美日韩二区三区-国产在线欧美一区日韩二区| 国产精品成人欧美激情-黄色床上完整版高清无遮挡| 久久99国产综合精品女人-日韩一区二区三区在线不卡| 亚洲美女喘息呻吟的网站-国产免费一区二区三区三洲| 蜜臀一区二区三区精品在线-99久久久精品免费看国产| 三级a级一级大片在线观看-日韩av有码免费观看| 亚洲av专区在线观看国产-丰满人妻av一区二区三区| 色噜噜噜噜一区二区三区-欧美最猛黑人做爰视频| 国产欧美一区二区三区嗯嗯-欧美一区二区日本国产激情| 婷婷亚洲欧美综合丁香亚洲-超刺激国语对白在线视频| 深夜三级福利在线播放-日韩精品一区二区在线天天狠天| 俄罗斯胖老太太黄色特级片-国产精品黑丝美腿美臀| 欧美看片一区二区三区-人妻无卡精品视频在线| 国产欧美日本一区二区-一区二区三区亚洲在线播放| 中文字幕社区电影成人-欧美精美视频一区二区三区| 亚洲区欧美区在线视频-亚洲碰碰人人AV熟女天堂| 国产欧美日韩中文字幕在线-国产伊人一区二区三区四区| 蜜臀av日日欢夜夜爽一区-av在线免费永久播放| 国产免费一区二区三区不-日本少妇免费一区二区三区| 久久高清超碰av热热久久-国产高清不卡免费视频| 亚洲另类自拍唯美另类-99国产精品兔免久久| 91大神国内精品免费网站-亚洲免费电影一区二区|